Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Flavokawain C 是存在卡瓦胡椒根中的一种天然查尔酮,具有发展成为治疗结肠腺癌的化疗药物的潜力。它对 HCT 116 细胞作用的 IC50值为 12.75 μM。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 283 | 现货 | ||
2 mg | ¥ 395 | 现货 | ||
5 mg | ¥ 662 | 现货 | ||
10 mg | ¥ 1,060 | 现货 | ||
25 mg | ¥ 1,930 | 现货 | ||
50 mg | ¥ 2,870 | 现货 | ||
100 mg | ¥ 4,170 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 762 | 现货 |
产品描述 | Flavokawain C (FKC), a naturally occurring chalcone, which can be isolated from Kava.FKC has the potential to be developed into chemotherapeutic drug for the treatment of colon adenocarcinoma. |
体外活性 | Flavokawain C (FKC) markedly decreased the cell viability of HT-29 cells and the cells showed dramatic changes in cellular and nuclear morphologies with typical apoptotic features.?The induction of apoptosis correlated well with the externalization of phosphatidylserine, DNA fragmentation, decreased mitochondrial membrane potential, activation of caspases, and PARP cleavage.?This was associated with an increase in reactive oxygen species and a decrease in SOD activity.?The protein levels of XIAP, c-IAP1, and c-IAP2 were downregulated, whereas the GADD153 was upregulated after FKC treatment.?FKC induced cell cycle arrest at the G1 and G2/M phases via upregulation of p21 and p27 in a p53-independent manner.??FKC has the potential to be developed into chemotherapeutic drug for the treatment of colon adenocarcinoma[1]. |
细胞实验 | Cell viability of HT-29 cells was assessed by Sulforhodamine B assay after FKC treatment.?Induction of apoptosis was examined by established morphological and biochemical assays.?ROS generation was determined by dichlorofluorescein fluorescence staining, and superoxide dismutase activity was measured using the spectrophotometric method.?Western blotting was used to examine the changes in the protein levels[1]. |
别名 | 黄卡瓦胡椒素 C, 卡瓦胡椒素C |
分子量 | 300.31 |
分子式 | C17H16O5 |
CAS No. | 37308-75-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 25 mg/mL (83.25 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 3.3299 mL | 16.6495 mL | 33.2989 mL | 83.2473 mL |
5 mM | 0.666 mL | 3.3299 mL | 6.6598 mL | 16.6495 mL | |
10 mM | 0.333 mL | 1.6649 mL | 3.3299 mL | 8.3247 mL | |
20 mM | 0.1665 mL | 0.8325 mL | 1.6649 mL | 4.1624 mL | |
50 mM | 0.0666 mL | 0.333 mL | 0.666 mL | 1.6649 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Flavokawain C 37308-75-1 Apoptosis 黄卡瓦胡椒素 C 卡瓦胡椒素C inhibit Inhibitor inhibitor